Worst Decision of My Life...well, almost.

My PI on vacation, Day 2.

Hmmph. So I'm trying to grow these mouse fetal liver cells to compare their promoter structure with that in RAW macrophages. These 'null' cells are wicked hard to grow up, I'm discovering. I received them on Thursday via FedEx, put them in fresh media, and they doubled overnight. I used them for a quickie experiment, then cut them back for the weekend. What happens Monday but I discover that over half of them are dead or dying. I combined the two remaining flasks and put the surviving cells in fresh media to grow overnight. Next morning - they're still dying. CRAP!!! I hate these cells.

These experiments better not be crucial to my finishing my project - I feel like quitting. This is so stupid - to be held hostage in lab by a bunch of freaking fickle cells. What is all this good for, anyway?

Are my ChIPs back up and running? Dunno. Check back later.

One good thing - looks like I can extract RNA again. Don't know what went wrong - just used mostly new solutions? Just one of those things.

Things aren't working too well right now. I'm feeling the pressure. My PI's feeling the pressure. OMG research sucks. Worst career choice in my life.

Mike